Shengjiang San alleviated sepsis-induced lung injury through its bidirectional regulatory effect.

Apr 17, 2023

Experts: Shifan Yan,Yu Jiang,Ting Yu,Changmiao Hou,Wen Xiao,Jing Xu,Huili Wen,Jingjing Wang,Shutong Li,Fang Chen,Shentang Li,Xiehong Hao Tan Liu,Lianhong Zou,Yanjuan Liu,Yimin Zhu

Sepsis is a life-threatening organ dysfunction caused by dysregulated host responses to infection, for which effective therapeutic strategies are still absent. Shengjiang San (SJS), a well-known Traditional Chinese Medicine formula, has been widely used clinically. However, its role in sepsis-induced lung injury remains unclear.
To explore its specific mechanism, we firstly established a sepsis animal model using cecal ligation and puncture (CLP) and treated MH-S cells with LPS plus ATP. Then, UPLC/Q-TOF-MS/MS was utilized to identify its active ingredients. Network pharmacology analysis was performed to uncover the potential mechanism. HE staining and biochemical analysis were conducted to validate its therapeutic effect. ELISA was applied to detect the release of pro-inflammatory and anti-inflammatory cytokines. Western blot was utilized to detect the protein levels of GSDMD, NLRP3, P65, ASC and caspase-1.
SJS could dramatically increase the survival rate of sepsis. In addition, it is able to inhibit the pro-inflammatory cytokines release at day 1 post CLP while promote their production at day 7, indicating SJS could attenuate uncontrolled inflammatory response in the early stage and improve immunosuppression in the late phase. Network pharmacology analysis showed that pyroptosis is the crucial action SJS exerted in the protection of sepsis-induced lung injury. Western blot data implicated SJS could attenuate pyroptosis in early sepsis while enhance in the late phase.
SJS acted to alleviate sepsis-induced lung injury through its bidirectional regulatory effect.

© 2023. The Author(s).

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ABOUT THE EXPERTS

Shifan Yan,Yu Jiang,Ting Yu,Changmiao Hou,Wen Xiao,Jing Xu,Huili Wen,Jingjing Wang,Shutong Li,Fang Chen,Shentang Li,Xiehong Hao Tan Liu,Lianhong Zou,Yanjuan Liu,Yimin Zhu

Shifan Yan

Department of Emergency, Institute of Emergency Medicine, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), 69 Jiefang Western Road, Changsha, 410000, Hunan, People’s Republic of China.

Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Changsha, Hunan, China.

Hunan University of Chinese Medicine, Changsha, Hunan, China.

Yu Jiang

Department of Emergency, Institute of Emergency Medicine, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), 69 Jiefang Western Road, Changsha, 410000, Hunan, People’s Republic of China.

Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Changsha, Hunan, China.

Ting Yu

Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, China.

Changmiao Hou

Hunan University of Chinese Medicine, Changsha, Hunan, China.

Wen Xiao

Department of Emergency, Institute of Emergency Medicine, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), 69 Jiefang Western Road, Changsha, 410000, Hunan, People’s Republic of China.

Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Changsha, Hunan, China.

Jing Xu

Department of Emergency, Institute of Emergency Medicine, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), 69 Jiefang Western Road, Changsha, 410000, Hunan, People’s Republic of China.

Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Changsha, Hunan, China.

Huili Wen

Department of Emergency, Institute of Emergency Medicine, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), 69 Jiefang Western Road, Changsha, 410000, Hunan, People’s Republic of China.

Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Changsha, Hunan, China.

Hunan University of Chinese Medicine, Changsha, Hunan, China.

Jingjing Wang

Department of Emergency, Institute of Emergency Medicine, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), 69 Jiefang Western Road, Changsha, 410000, Hunan, People’s Republic of China.

Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Changsha, Hunan, China.

Shutong Li

Department of Emergency, Institute of Emergency Medicine, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), 69 Jiefang Western Road, Changsha, 410000, Hunan, People’s Republic of China.

Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Changsha, Hunan, China.

Fang Chen

Department of Emergency, Institute of Emergency Medicine, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), 69 Jiefang Western Road, Changsha, 410000, Hunan, People’s Republic of China.

Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Changsha, Hunan, China.

Shentang Li

Department of Pediatrics, Third Xiangya Hospital of Central South University, Changsha, Hunan, China.

Xiehong Hao Tan Liu

Department of Emergency, Institute of Emergency Medicine, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), 69 Jiefang Western Road, Changsha, 410000, Hunan, People’s Republic of China.

Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Changsha, Hunan, China.

Lianhong Zou

Department of Emergency, Institute of Emergency Medicine, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), 69 Jiefang Western Road, Changsha, 410000, Hunan, People’s Republic of China.

Yanjuan Liu

Department of Emergency, Institute of Emergency Medicine, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), 69 Jiefang Western Road, Changsha, 410000, Hunan, People’s Republic of China. liuyanjuanhunan@163.com.

Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Changsha, Hunan, China. liuyanjuanhunan@163.com.

Yimin Zhu

Department of Emergency, Institute of Emergency Medicine, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), 69 Jiefang Western Road, Changsha, 410000, Hunan, People’s Republic of China. cs_zhuyimin@163.com.

Hunan Provincial Key Laboratory of Emergency and Critical Care Metabonomics, Changsha, Hunan, China. cs_zhuyimin@163.com.

Hunan University of Chinese Medicine, Changsha, Hunan, China. cs_zhuyimin@163.com.