This study states that The point of the current investigation was to decide, by histological and stereological examination, regardless of whether there are between-sex primary contrasts in the bladder in the second gestational trimester in human embryos.
Forty bladders, which were gotten from 40 human babies (20 guys and 20 females) going in age from 13 to 23 weeks post-origination (WPC), were examined. The babies were visibly very much protected, without inconsistencies of the urinary and genital frameworks; the cases with disorder were deserted. The bladders were analyzed and installed in paraffin, from which 5-μm thick segments were gotten and stained with: Masson’s trichrome, to evaluate connective and smooth muscle tissue; Weigert’s resorcin fuchsin, to notice versatile filaments; picrosirius red with polarization, to notice collagen; and against beta III tubulin counter acting agent, to notice the bladder nerves. The hatchlings weighed somewhere in the range of 60 and 490 g, and had crown–back end lengths somewhere in the range of 9.5 and 20.4 cm. No flexible framework filaments were seen in any bladders. The histological examination of the smooth muscle, collagen, nerves and connective tissue of the creating bladders uncovered that there are no sexual orientation contrasts during weeks 13–23 of incubation.
Reference link- https://www.sciencedirect.com/science/article/abs/pii/S1477513114003052
