Under the condition of LPS/IFN-γ activation, macrophage metabolism is conversed from oxidative phosphorylation to glycolysis. In the present work, we analyzed whether glycolysis could affect IL-1β expression through altering histone acetylation level in mouse bone marrow-derived macrophages. Immunocytochemistry and Western blot analysis are used to characterize histone acetylation in macrophages stimulated by LPS/IFN-γ. RT-PCR and ELISA were used to determine IL-1β production. Macrophages’ metabolism was monitored in real time by Seahorse test. Our results showed that glycolytic metabolism could enhance the histone acetylation and promote the IL-1β production in LPS/IFN-γ activated macrophage. Moreover, increased production of IL-1β by glycolysis was mediated through enhanced H3K9 acetylation. Importantly, it is found that high-dose of HDAC inhibitor could also significantly increase the expression of IL-1β in the absence of glycolytic metabolism. In conclusion, this study demonstrates that glycolytic metabolism could regulate IL-1β expression by increasing histone acetylation levels in LPS/IFN-γ stimulated macrophages.This article is protected by copyright. All rights reserved.
