Photo Credit: tumeyes
The following is a summary of “Effect of tumour necrotic factor-α, interleukin-17 and interleukin-22 on the expression of filaggerin-2 and hornerin: Analysis of a three-dimensional psoriatic skin model,” published in the September 2024 issue of Dermatology by Makino et al.
Filaggrin-2 (FLG2) and hornerin (HRNR), like filaggrin (FLG), are members of the S100 fused-type protein family, with FLG2 expression decreased in psoriatic skin, while HRNR expression varies.
Researchers conducted a retrospective study examining the influence of TNF-α, IL-17A, and IL-22 on FLG2 and HRNR expression in a 3D psoriatic skin model.
They generated a 3D psoriatic skin model and stimulated it with TNF-α, IL-17A, and IL-22 for 3D skin equivalents. The FLG2 and HRNR expression changes were examined via quantitative reverse transcription polymerase chain reaction and immunostaining.
The results showed that in the 3D psoriatic skin model, FLG2 and HRNR expressions were significantly lower than in the 3D control skin. Stimulation with TNF-α, IL-17A, and IL-22 notably reduced FLG2 levels. Conversely, IL-22 stimulation markedly increased HRNR expression, whereas TNF-α and IL-17A slightly decreased relative to the 3D control skin.
Investigators concluded that TNF-α, IL-17A, and IL-22 differentially affected FLG2 and HRNR expression and epidermal structure formation, suggesting distinct roles in barrier formation and psoriasis pathogenesis.
Source: onlinelibrary.wiley.com/doi/full/10.1002/ski2.440
