Cofilin-1 promotes fibrocyte differentiation and contributes to pulmonary fibrosis.

Jun 7, 2021

Contributor: Wei Guo,Ting Guo,Qianhui Zhou,Yaomei Long,Man Luo,Qinxue Shen,Wang Duan,Xiaoli Ouyang,Hong Peng

Fibrocytes originate from the bone marrow monocyte lineage and participate in the pathogenesis of pulmonary fibrosis. Research providing a comprehensive picture of fibrocytes is still limited. Cofilin-1 (CFL-1) is an important protein that regulates cell proliferation, migration and differentiation. Whether CFL-1 can induce monocyte differentiation into fibrocytes and promote the process of pulmonary fibrosis is unknown. Compared with that of healthy controls, the expression of CFL-1 was significantly increased in the plasma and peripheral blood mononuclear cells (PBMCs) from idiopathic pulmonary fibrosis (IPF) and connective tissue disease-associated interstitial lung disease (CTD-ILD) patients (P < 0.05). The percentages of peripheral blood fibrocytes in the IPF group (4.2550 ± 0.3483%) and CTD-ILD group (4.7100 ± 0.4811%) were higher than that in the control group (1.6340 ± 0.2549%) (both P < 0.05). In vitro, PBMCs transfected with siRNA-CFL-1 showed lower expression of CFL-1, and the percentage of fibrocytes was lower than that of the control (P < 0.05). PBMCs transfected with Lv-CFL-1 to increase the expression of CFL-1 showed a higher percentage of fibrocytes than the control (P < 0.05). In mice with bleomycin-induced pulmonary fibrosis, the relative expression of CFL-1 was increased, and the percentage of fibrocytes was higher than that in the saline group (P < 0.05). In bleomycin-induced mice, interference with Lv-CFL-1 decreased the expression of CFL-1, the percentage of fibrocytes was lower, and the lung tissue showed less fibrosis (P < 0.05). The overexpression of CFL-1 is associated with pulmonary fibrogenesis. CFL-1 could promote the differentiation of fibrocytes from monocyte peripheral blood mononuclear cells and promote pulmonary fibrosis.
Copyright © 2021 Elsevier Inc. All rights reserved.

Author

admin

View all posts

About The Expert

Wei Guo

Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central-South University, Changsha, Hunan, China; Research Unit of Respiratory Disease, Central-South University, Changsha, Hunan, China; The Respiratory Disease Diagnosis and Treatment Center of Hunan Province, Changsha, Hunan, China.

Ting Guo

Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central-South University, Changsha, Hunan, China; Research Unit of Respiratory Disease, Central-South University, Changsha, Hunan, China; The Respiratory Disease Diagnosis and Treatment Center of Hunan Province, Changsha, Hunan, China.

Qianhui Zhou

Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central-South University, Changsha, Hunan, China; Research Unit of Respiratory Disease, Central-South University, Changsha, Hunan, China; The Respiratory Disease Diagnosis and Treatment Center of Hunan Province, Changsha, Hunan, China; Second Department of Respiratory and Critical Care Medicine, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, CSU, Zhuzhou, Hunan, China.

Yaomei Long

Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central-South University, Changsha, Hunan, China; Research Unit of Respiratory Disease, Central-South University, Changsha, Hunan, China; The Respiratory Disease Diagnosis and Treatment Center of Hunan Province, Changsha, Hunan, China.

Man Luo

Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central-South University, Changsha, Hunan, China; Research Unit of Respiratory Disease, Central-South University, Changsha, Hunan, China; The Respiratory Disease Diagnosis and Treatment Center of Hunan Province, Changsha, Hunan, China.

Qinxue Shen

Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central-South University, Changsha, Hunan, China; Research Unit of Respiratory Disease, Central-South University, Changsha, Hunan, China; The Respiratory Disease Diagnosis and Treatment Center of Hunan Province, Changsha, Hunan, China.

Wang Duan

Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central-South University, Changsha, Hunan, China; Research Unit of Respiratory Disease, Central-South University, Changsha, Hunan, China; The Respiratory Disease Diagnosis and Treatment Center of Hunan Province, Changsha, Hunan, China.

Xiaoli Ouyang

Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central-South University, Changsha, Hunan, China; Research Unit of Respiratory Disease, Central-South University, Changsha, Hunan, China; The Respiratory Disease Diagnosis and Treatment Center of Hunan Province, Changsha, Hunan, China.

Hong Peng

Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central-South University, Changsha, Hunan, China; Research Unit of Respiratory Disease, Central-South University, Changsha, Hunan, China; The Respiratory Disease Diagnosis and Treatment Center of Hunan Province, Changsha, Hunan, China. Electronic address: penghong66@csu.edu.cn.

References

PubMed