Ischemia on the colon wall negatively affects healing of anastomosis. We were aimed to evaluate the effects of Carbon Monoxide Releasing Molecule-2 (CORM-2) on the healing of anastomosis in a rat model of the ischemic colon.
A total of 60 rats were randomly divided into three groups as colon transection and end-to-end anastomosis (Group I), colon transection and end-to-end anastomosis following the induction of ischemia (Group II), and colon transection and end-to-end anastomosis following the induction of ischemia and treated with daily intraperitoneal administration of CORM-2 (Group III). Each group was also divided into two equal subgroups as postoperative 3rd and 7th day. Postoperative healing of anastomoses was evaluated by anastomosis burst pressure (ABP), tissue biomarkers including hydroxyproline (HP), malondialdehyde (MDA), glutathione (GSH), and histopathological findings.
In the ischemic group treated with CORM-2, lower MDA and higher HP levels were observed in comparison to the untreated ischemic group at the 3rd day. GSH and HP levels in the ischemic rats treated with CORM-2 were higher than in the ischemic untreated rats at the 7th day. In the ischemic group treated with CORM-2, the mucosal epithelial score decreased and the neoangiogenesis score increased compared to the untreated rats at 7th day.
CORM-2 reduces cell destruction by suppressing the oxidative reaction and strengthening the antioxidative mechanisms of the cells in ischemic colon anastomosis. It also increases collagen formation, epithelial development, and neoangiogenesis.

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