Probiotic Properties of Alcaligenes faecalis Isolated from Argyrosomus regius in Experimental Peritonitis (Rat Model).

Mar 15, 2021

Contributor: A I Gutiérrez-Falcón,A M Ramos-Nuez,A Espinosa de Los Monteros Y Zayas,D F Padilla Castillo,M Isabel García-Laorden,F J Chamizo-López,F Real Valcárcel,F Artilles Campelo,A Bordes Benítez,P Nogueira Salgueiro,C Domínguez Cabrera,J C Rivero-Vera,J M González-Martín,J Martín Caballero,R Frías-Beneyto,Jesús Villar,J L Martín-Barrasa

Probiotic Properties of Alcaligenes faecalis Isolated from Argyrosomus regius in Experimental Peritonitis (Rat Model).

A strain of Alcaligenes faecalis A12C (A. faecalis A12C) isolated from Argyrosomus regius is a probiotic in fish. Previous experiments showed that A. faecalis A12C had inhibitory effects on the growth of multidrug-resistant bacteria. We aimed to confirm whether A. faecalis A12C is safe and has adequate intestinal colonization in experimental rats, and evaluate its efficacy in an animal model of peritonitis. We used 30 male rats, randomly divided into 6 groups (n = 5): three groups (HA7, HA15, HA30) received A. faecalis A12C in drinking water (6 × 10 CFU/mL) for 7 days, and three control groups received drinking water only. All groups were evaluated at 7, 15, and 30 days. Survival after A. faecalis A12C administration was 100% in all groups. Mild eosinophilia (1.5%, p < 0.01) and increased aspartate aminotransferase (86 IU/L, p < 0.05) were observed in HA7, followed by progressive normalization. No histological signs of organ injury were found. We observed significant E. coli decline in faeces, parallel to an increase in A. faecalis A12C at 7 days. E. coli had a tendency to recover initial values, while A. faecalis A12C disappeared from the intestinal microbiota at 30 days. To evaluate its efficacy against peritonitis, we studied two additional groups of animals: IA group pretreated with A. faecalis A12C before E. coli intra-abdominal inoculation, and IC group inoculated with no A. faecalis A12C. We found an increase in C-reactive protein, alanine aminotransferase, urea, and eosinophils in IC animals when compared with IA. Peritonitis was more evident in IC than in IA animals. Our findings suggest that A. faecalis A12C altered clinically relevant parameters in sepsis and was associated with a lesser spread of infection.

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About The Expert

A I Gutiérrez-Falcón

Group of Fish Health and Infectious Diseases, Universitary Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, Carretera de Trasmontaña s/n, 35416, Arucas, Spain.

A M Ramos-Nuez

CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Monforte de Lemos 3-5, Pabellón 11, 28029, Madrid, Spain.

Multidisciplinary Organ Dysfunction Evaluation Research Network, Research Unit, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de la Ballena s/n, 35019, Las Palmas de Gran Canaria, Spain.

A Espinosa de Los Monteros Y Zayas

Morphology Department, Universitary Institute of Animal Health and Food Safety (IUSA), Universidad de Las Palmas de Gran Canaria. Arucas, Las Palmas, Spain.

D F Padilla Castillo

Group of Fish Health and Infectious Diseases, Universitary Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, Carretera de Trasmontaña s/n, 35416, Arucas, Spain.

M Isabel García-Laorden

CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Monforte de Lemos 3-5, Pabellón 11, 28029, Madrid, Spain.

Multidisciplinary Organ Dysfunction Evaluation Research Network, Research Unit, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de la Ballena s/n, 35019, Las Palmas de Gran Canaria, Spain.

F J Chamizo-López

Microbiology Department. Hospital,, Universitario de Gran Canaria Dr Negrín, Barranco de La Ballena S/N, 35019, Las Palmas de Gran Canaria, Spain.

F Real Valcárcel

Group of Fish Health and Infectious Diseases, Universitary Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, Carretera de Trasmontaña s/n, 35416, Arucas, Spain.

F Artilles Campelo

Microbiology Department. Hospital,, Universitario de Gran Canaria Dr Negrín, Barranco de La Ballena S/N, 35019, Las Palmas de Gran Canaria, Spain.

A Bordes Benítez

Microbiology Department. Hospital,, Universitario de Gran Canaria Dr Negrín, Barranco de La Ballena S/N, 35019, Las Palmas de Gran Canaria, Spain.

P Nogueira Salgueiro

Clinical Biochemistry Department, Hosital Universitario de Gran Canaria Dr Negrín, Barranco de La Ballena S/N, 35019, Las Palmas de Gran Canaria, Spain.

C Domínguez Cabrera

Clinical Biochemistry Department, Hosital Universitario de Gran Canaria Dr Negrín, Barranco de La Ballena S/N, 35019, Las Palmas de Gran Canaria, Spain.

J C Rivero-Vera

Pathology Service. Hospital, Universitario de Gran Canaria Dr Negrín, Barranco de La Ballena S/N, 35019, Las Palmas de Gran Canaria, Spain.

J M González-Martín

Statistics Service. Research Unit, Hospital Universitario de Gran Canaria Dr Negrín, Barranco de La Ballena S/N, 35019, Las Palmas de Gran Canaria, Spain.

J Martín Caballero

Barcelona Biomedical Research Park (PRBB), Barcelona, Spain.

R Frías-Beneyto

Comparative Medicine, Karolinska Institutet, Stockholm, Sweden.

Jesús Villar

CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Monforte de Lemos 3-5, Pabellón 11, 28029, Madrid, Spain.

Multidisciplinary Organ Dysfunction Evaluation Research Network, Research Unit, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de la Ballena s/n, 35019, Las Palmas de Gran Canaria, Spain.

J L Martín-Barrasa

Group of Fish Health and Infectious Diseases, Universitary Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, Carretera de Trasmontaña s/n, 35416, Arucas, Spain. joseluis.martin@ulpgc.es.

Multidisciplinary Organ Dysfunction Evaluation Research Network, Research Unit, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de la Ballena s/n, 35019, Las Palmas de Gran Canaria, Spain. joseluis.martin@ulpgc.es.

Animal Facility, Research Unit, Hospital Universitario de Gran Canaria Dr Negrín, Barranco de La Ballena S/N, 35019, Las Palmas de Gran Canaria, Spain. joseluis.martin@ulpgc.es.

References

PubMed